Journal: Oxidative medicine and cellular longevity

Article Title: Mitochondrial-Targeting Antioxidant SS-31 Suppresses Airway Inflammation and Oxidative Stress Induced by Cigarette Smoke.

doi: 10.1155/2021/6644238

Figure Lengend Snippet: Figure 6: SS-31 prevents mitochondrial dysfunction in CS-induced mouse lung. (a–d) Protein levels of OPA1, MFF (a, b), and cytochrome c (c, d) in lungs were evaluated by western blot following exposure to CS with or without SS-31 pretreatment. GAPDH or COX IV was used as a loading control (n = 3 per group). ∗∗P < 0:01 and ∗∗∗P < 0:001 vs. control; #P < 0:05 vs CS. Abbreviations: COX IV: cytochrome c oxidase IV; CS: cigarette smoke; Cyt c: cytochrome c; GAPDH: glyceraldehyde 3-phosphate dehydrogenase; MFF: mitochondrial fission factor; mit: mitochondrion; OPA1: optic atrophy 1; SS-31 (H): high-dose SS-31 (5 mg/kg); SS-31 (L): low-dose SS-31 (2.5 mg/kg).

Article Snippet: After blocking with 5% bovine serum albumin in Trisbuffered saline at room temperature for 1 h, the membrane was incubated overnight at 4°C with antibodies against optic atrophy (OPA) 1 (mouse antibody from Cell Signaling Technology; human antibody from Proteintech, Rosemont, IL, USA); mitochondrial fission factor (MFF) (mouse antibody from Cell Signaling Technology; human antibody from Proteintech); and extracellular signal-regulated kinase (ERK), phosphorylated- (P-) ERK, P38, P-P38, and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (all from Cell Signaling Technology).

Techniques: Western Blot, Control

Journal: Oxidative medicine and cellular longevity

Article Title: Mitochondrial-Targeting Antioxidant SS-31 Suppresses Airway Inflammation and Oxidative Stress Induced by Cigarette Smoke.

doi: 10.1155/2021/6644238

Figure Lengend Snippet: Figure 11: SS-31 protects against CSE-induced mitochondrial dysfunction via suppression of MAPK signaling. (a, b) Protein levels of OPA1 and MFF detected by western blot in BEAS-2B cells (n = 3 per group). ∗∗P < 0:01, ∗∗∗P < 0:001, and ∗∗∗∗P < 0:0001 vs. control; #P < 0:05 and ##P < 0:01 vs. ani+CSE; &P < 0:05 vs. CSE. Abbreviations: ani: anisomycin; CSE: cigarette smoke extract; MFF: mitochondrial fission factor; OPA1: optic atrophy 1.

Article Snippet: After blocking with 5% bovine serum albumin in Trisbuffered saline at room temperature for 1 h, the membrane was incubated overnight at 4°C with antibodies against optic atrophy (OPA) 1 (mouse antibody from Cell Signaling Technology; human antibody from Proteintech, Rosemont, IL, USA); mitochondrial fission factor (MFF) (mouse antibody from Cell Signaling Technology; human antibody from Proteintech); and extracellular signal-regulated kinase (ERK), phosphorylated- (P-) ERK, P38, P-P38, and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (all from Cell Signaling Technology).

Techniques: Western Blot, Control

Journal: Oxidative Medicine and Cellular Longevity

Article Title: 5-Azacytidine and Resveratrol Enhance Chondrogenic Differentiation of Metabolic Syndrome-Derived Mesenchymal Stem Cells by Modulating Autophagy

doi: 10.1155/2019/1523140

Figure Lengend Snippet: Evaluation of mitochondrial dynamics during chondrogenic differentiation. Prior to experiments, cells were pretreated with AZA/RES for 24 hours, then the experimental medium was replaced by osteogenic differentiation medium. Cells were cultured in that medium for five days and after were subjected to further analysis. Mitochondrial morphology was visualized using TEM (a). Mitochondria from ASC EMS were characterized by disarrayed cristae and vacuole formation. Furthermore, expression of FIS (b) and MFN (c) was evaluated using RT-PCR. Amount of MFF and MFN protein cell lysates was established with western blot (d). Abbreviations: Mt: mitochondria, ER: endoplasmic reticulum, V: vacuole. Results expressed as mean ± S.D. Statistical significance indicated as an asterisk ( ∗ ) when comparing the result to ASC EMS and as a number sign (#) when comparing to ASC CTRL . ∗ p < 0.05; ### and ∗∗∗ p < 0.001.

Article Snippet: Afterwards, the membranes were washed with TBST and incubated with primary antibody against β -actin (Sigma-Aldrich, A5441), mitofusin-1 (MFN-1) (Biorbyt, orb11040,), and mitochondrial fission factor (MFF) (Biorbyt orb325479) at a dilution of 1 : 500 for 2 hours followed by incubation with ALP-conjugated secondary antibodies.

Techniques: Cell Culture, Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot